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The XMG1.2 monoclonal antibody reacts with mouse IFNγ (interferon gamma) a 20 kDa soluble pleiotropic cytokine and the sole member of the type II class of interferons. IFNγ is primarily produced by activated lymphocytes including T, B, NK cells, and ILCs. IFNγ exerts immunoregulatory, anti-proliferative, anti-viral, and proinflammatory activities and plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. Additionally, IFNγ induces the production of cytokines, Fc receptor, and adhesion molecules and up-regulates MHC class I and II antigen expression by antigen presenting cells during an immune response. IFNγ has also been shown to modulate macrophage effector functions, influence isotype switching and induce the secretion of immunoglobulins by B cells. IFNγ signals through the IFN gamma receptor which exists as a heterodimer composed of CD119 (IFN gamma receptor 1) and AF-1 (IFN gamma receptor 2). The IFNγ receptor is expressed ubiquitously on almost all cell types with the exception of mature erythrocytes. The XMG1.2 antibody is a neutralizing antibody.

The GK1.5 monoclonal antibody reacts with the mouse CD4. The CD4 antigen is a 55 kDa cell surface type I membrane glycoprotein belonging to the immunoglobulin superfamily. CD4 acts as a co-receptor which in cooperation with the T cell receptor (TCR) interacts with class II MHC molecules displayed by antigen presenting cells (APC). CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally. The GK1.5 antibody has been shown to compete with clones YTS 177 and YTS 191 for CD4 binding.

The YTS 191 monoclonal antibody reacts with mouse CD4. The CD4 antigen is a 55 kDa type I cell-surface membrane glycoprotein belonging to the immunoglobulin superfamily. CD4 acts as a co-receptor which in cooperation with the T cell receptor (TCR) interacts with class II MHC molecules displayed by antigen presenting cells (APC). CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally. The YTS 191 antibody has been shown to compete with clones GK1.5 and YTS 177 for CD4 binding.

The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.

The 1A8 monoclonal antibody reacts with mouse Ly6G. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. Unlike the RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.

The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.

The P3-7B monoclonal antibody reacts with human carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM7). CEACAMs are highly glycosylated membrane proteins belonging to the immunoglobulin superfamily. The function of CEACAM7 still has to be elucidated but it has been described to be expressed in columnar epithelial cells of the large intestine and strongly downregulated in colonic adenocarcinomas. Antibodies against CEACAMs are commonly used in immunohistochemistry and flow cytometry to identify cells expressing the glycoprotein in tissue samples and cell culture.

The M1/69 monoclonal antibody reacts with mouse CD24 also known as heat stable antigen (HSA), a 35-50 kDa glycosylated membrane protein. CD24 is expressed by erythrocytes, granulocytes, monocytes, lymphocytes, and dendritic cells. Immature B cells in bone marrow and spleen of adult mice express high levels of CD24, while mature peripheral B cells express intermediate levels of CD24. The expression of CD24 has been used to resolve stages of B cell lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is often overexpressed in human tumors. In cancer, CD24 is a regulator of cell migration, invasion, and proliferation.

The 6G5j monoclonal antibody reacts with an antigen epitope shared by mouse carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 1, 3, 5, 6, and 8, also known as CD66a, d, e, c, and b. CEACAMs are highly glycosylated membrane proteins belonging to the immunoglobulin superfamily. CEACAMs are involved in cell adhesion, proliferation, differentiation, apoptosis, T and B cell activation and except for CEACAM 8, they also serve as bacterial pathogen receptors. CEACAM 1 is expressed by epithelial, endothelial, and immune cells. CEACAM 3 and 8 are solely expressed in granulocytes, CEACAM 5 in epithelial cells and CEACAM 6 in epithelium and granulocytes. Antibodies against CEACAMs are commonly used in immunohistochemistry and flow cytometry to identify cells expressing the glycoprotein in tissue samples and cell culture. However, CEACAM 1, 5 and 6 are also found in serum where they can be used as tumor markers.

The 9E9 monoclonal antibody reacts with mouse CD16.2, also known as FcγRIV (Fc receptor, IgG, low affinity IV). CD16.2 is a member of the immunoglobulin superfamily and is expressed on monocytes, macrophages, dendritic cells, and neutrophils. Fcγ receptors are essential for IgG-dependent effector functions in vivo. CD16.2 requires the common Fcγ chain for expression and signaling. CD16.2 binds to IgG2a and IgG2b with intermediate affinity. IgG2a- and IgG2b-dependent effector functions are severely impaired in CD16.2 deficient mice. CD16.2 has also been reported to be a low-affinity IgE receptor for all IgE allotypes and promotes IgE-induced lung inflammation. The 9E9 antibody has been shown to inhibit cellular CD16.2 function.

The MOAB-2 monoclonal antibody (epitope mapped to residues 1-5 of human Aβ) is a pan-specific antibody. Using synthetic Aβ, it recognizes unaggregated, oligomeric or fibrillar forms of Aβ42 and unaggregated Aβ40, and is selective for human Aβ42 over Aβ40. With synthetic Aβ or human or rodent brain homogenates, MOAB-2 specifically detects Aβ, but not amyloid precursor protein (APP), which is cleaved by β- and Y- secretase to release Aβ. With immunostaining of human or rodent tissue, MOAB-2 detects intraneuronal Aβ, as well as diffuse or compact amyloid plaques.

培养基C6366 - 500ML
EX - CELL® CHO Cloning Medium
Animal - component free. without L-glutamine, liquid, sterile-filtered, suitable for cell culture

The RMP1-14 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells.

The 53-6.7 monoclonal antibody reacts with mouse CD8α. The CD8 antigen is a transmembrane glycoprotein that acts as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. CD8 most commonly exists as a heterodimer composed of one CD8α and one CD8β chain however, it can also exist as a homodimer composed of two CD8α chains. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The molecular weight of each CD8 chain is approximately 34 kDa. The 53-6.7 antibody exhibits depleting activity when used in vivo.

The 145-2C11 monoclonal antibody reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The 145-2C11 antibody has been shown to induce T lymphocyte activation, proliferation, and apoptosis via binding and stimulating the TCR. Additionally, the 145-2C11 antibody has been reported to block the binding of the 17A2 antibody to CD3ε+ T lymphocytes.