InVivoPlus anti-mouse CD25 (IL-2Rα)
Cat.#
BP0012
规格
1mg
The PC-61.5.3 monoclonal antibody reacts with mouse IL-2Rα also known as CD25, Ly-43, p55, or Tac. IL-2Rα is the 55 kDa ligand-binding subunit of the interleukin 2 receptor alpha chain. IL-2Rα is expressed on activated mature T and B lymphocytes, thymocyte subsets, pre-B cells, and T regulatory cells. IL-2Rα has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Alone, the IL-2Rα binds IL-2 with relatively low affinity however, when IL-2Rα associates with IL-2Rβ (CD122) and the common gamma chain (CD132) the complex binds IL-2 with high affinity. The PC-61.5.3 antibody has been shown to inhibit the binding of IL-2 to both the low and high affinity IL-2 receptor forms. Additionally, the PC-61.5.3 antibody is commonly used to deplete CD4+FoxP3+ T regulatory cells in vivo.
概述
| Isotype | Rat IgG1, λ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus rat IgG1 isotype control, anti-horseradish peroxidase |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | IL-2-dependent cytolytic mouse T cell clone B6.1 |
| Reported Applications | in vivo regulatory T cell depletion Flow cytometry |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin* | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
| Aggregation* | <5% Determined by SEC |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 μM filtered |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A High Salt |
| RRID | AB_1107619 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests* | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
应用
储存
4°C
